39. infoExpo 2006 à Lausanne - Séminaire, Workshop et Exposition

Lieu de séminaire & d'exposition: Hôtel Mövenpick

 

Programme Séminaire

BPX90, a new polar Phase - for what?
Bernd Pfeffer, SGE (Deutschland) GmbH/infochroma ag

SGE has develloped a new polar phase with very interesting results in different application fields. This is based on a special selectivity, to which (pi)-(pi) interactions and H-bonding deliver the main contributions. This gives in a lot of cases different elution orders, which can be of great analytical value.

La GC olfactometrique: Le raccord entre GC ou GC/MS et le nez humain
Dr. Robert Stoop, Brechbühler AG

Technologie actuelle et applications dans différents secteurs (Environnement, Alimentaires, Parfumes et Arômes)

Headspace à Trappe et Désorbeur Thermique de nouvelle génération: leurs complémentarités et applications pratiques
Denis Rousseil, PerkinElmer AG

Nous passerons en revue les principes et technologies impliquées dans ces deux systèmes de plus en plus répandus. Au travers d’applications variées (environnement, chimie fine, pharmaceutique, etc..), nous découvrions leurs possibilités concrètes, leurs points communs, leurs spécificités et ce qu’ils peuvent vous apporter dans la vie de tous les jours au laboratoire.

HPTLC a modern analytical tool
Dr. Anita Ankli, CAMAG

Currently the interest in HPTLC, the high-performance version of thin-layer chromatography is surging. Especially in the field of quality control of botanicals, dietary supplements, and medicinal plants the technique became indispensable. For a long period of time TLC has been regarded as simple, outdated, and crude, but recent years have seen dramatic improvements of technology, theoretical concepts and applications. Standardized approaches and validated methods in combination with state of the art instrumentation allow reproducible and reliable work in a GxP environment.

Fast and fastest HPLC columns and instruments in direct comparison
Daniel Zimmerli, F. Hoffmann-La Roche AG

Ces derniers mois, le marché de l’ HPLC ultra rapide a beaucoup évolué. Avec les derniers modèles vous obtenez en un temps record des chromatogrammes avec une meilleure résolution. Actuellement, nos analyses en « open access » sont de l’ordre de 5 min : avec plus de 100 mesures quotidienne, les journées sont trop courtes.
Je vais essayer de vous montrer comment vous pouvez obtenir des séparations de 1 min environ avec ces nouveaux appareils et ceci sans grands réajustements des méthodes
Les colonnes se sont adaptées à ce nouveau marché. Ainsi nous trouvons des colonnes dont la granulométrie est bien inférieure à 2 µm dans des dimensions variables. Nous avons testé plusieurs nouvelles colonnes que nous vous avons comparé avec celles installées chez nous. Aussi bien avec les nouvelles colonnes qu’avec les traditionnelles dont la granulométrie varient entre 2, 5 et 5 µm, vous obtenez des séparations rapides.

Nouvelles approches en analyse des protéines avec des colonnes HIC-, IMAC- et monolithiques nouvellement développées
Fabrizio Sabini, Dionex (Switzerland) AG

Dionex a introduit de nouvelles colonnes HIC-, IMAC- et monolithiques lors de la Pittcon 2006. Les avantages de ces produits sont montrés à l’aide d’exemples par rapport aux produits existants. Les colonnes HIC et IMAC sont avant tout destinées aux chimistes des protéines qui recherchent une pureté plus élevée des protéines (ex. protéines recombinantes) et qui doivent analyser des mélanges complexes de protéines de façon rapide et reproductible. L’utilisation de colonnes monolithiques permet de réduire considérablement le temps d’analyse pour des digestions tryptiques d’échantillons pré purifiés. Cette technique de séparation se distingue par une haute efficacité de séparation même à des flux très élevés ainsi qu’une haute capacité tout en maintenant la contre-pression basse.

 

Workshops

Increased detection limit with ITEX
Bernhard Fischer, BGB Analytik AG

BGB has developed in cooperation with CTC a new technique for sample enrichment - used for Headspace applications. This new technique - ITEX - allows an enrichment of a gas phase on a solid support, which can be desorbed directly into an injector of a GC. This enrichment allows an increased sensitivity, because the loading is nearly endless. We will show some applications and the limitation of this new technique

Le Choix des colonnes HPLC aujourd‘hui
Roger Simon, Brechbühler AG

Le nombre de colonnes HPLC nécessaire peut être réduit de manière radicale par un habile choix des phases. Quelles .... colonnes sont nécessaires et pour quelles
applications ? Un aperçu de la philosophie des colonnes Phenomenex.

Reproducible HPTLC through standardized methods and modern instrumentation
Jürg Leuenberger, CAMAG

The quality of results in HPTLC can be affected by numerous parameters which give great flexibility to the technique. When reproducibility counts, as it is the case in quality control, most parameters should be kept constant and independent of human skills and errors. CAMAG introduces two new instruments for HPTLC. The Automatic developing chamber ADC 2 gives complete control over the development process. Chamber saturation, developing distance and the drying step as well as activity of the HPTLC plate are controlled and guarantee the same result day in and day out. With the documentation system DigiStore 2 images of the HPTLC Chromatogram can be captured in less than a second, keeping all essential parameters under complete control and independent of personal photographic skills.

Nouvelles possibilités de la chromatographie en deux dimensions HPLC (2D-LC) avec l’ULTIMATE 3000 Dual Gradient System: exemples dans le domaine de l’analyse des protéines
Roland Locher, Dr. ès Sc., Dionex (Switzerland) AG

La chromatographie en deux dimensions est toujours plus utilisée par les chimistes des protéines dans le but de gagner des informations de haute qualité en un minimum de temps. Le logiciel CHROMELEON en combinaison avec les modules intelligents de l’ULTIMATE 3000 permet aussi à des utilisateurs moins expérimentés d’accomplir des analyses chromatographiques complexes avec peu de préparation.

Improving productivity in organic purification
Dr. Jörg Heerklotz / IG Instrumenten-Gesellschaft AG

· Flash-Chromatography (FC)
· Gradients in FC
· Minimizing purification time with high resolution FC
FC purification of high pKa organic compounds with specialty RediSep® columns

Chromatographe préparatif et analytic en phase supercritique (SFC)
Timur Dogan, Mettler Toledo (Schweiz) AG

upercritical Fluid Chromatography has become the method of choice for many scientists working on the development of analytical and preparative processes. In comparison to HPLC in SFC we work with a supercritical liquid as the mobile phase. For practical reason in most cases CO2 has been used. Particularly for separating chiral compounds or as orthogonal technique to rp-HPLC, SFC possesses several benefits.
The components used in SFC are mostly the same as used in a HPLC System. You can use your existing normal phase columns. The main difference is the use of a supercritical liquid as the mobile phase. The reduction of organic solvent and the easy removal of the modifier (alcohol) to get your product make SFC a green technology. Less waste, lower energy consumption and time saving are the typical advantages of preparative SFC.
The physical properties of supercritical liquids give you several advantages in chromatography over normal liquids. The used liquid CO2 mixed with methanol (or another alcohol) as modifier has a lower viscosity, thus high flow-rate with a low pressure drop are possible and longer columns can be used. The speed of the diffusion of the mobile phase is between a gas and a liquid hence the re-equilibration of the column is done in seconds! The low viscosity and fast diffusion result in a 4 to 5 time faster separation compared to classical HPLC. If chiral compounds have to be separated it is an order of magnitude faster.
Very often analytical separation can be carried out within less than 2 minutes. This helps to substantial increase throughput and reduces the time for method development dramatically.

Zorbax Rapid Resolution High Throughput LC Column (RRHT) 1,8 µm
Joyce Link, Milian SA

Headspace à Trappe et Désorbeur Thermique de nouvelle génération: leurs complémentarités et applications pratiques
Denis Rousseil, PerkinElmer AG

Nous passerons en revue les principes et technologies impliquées dans ces deux systèmes de plus en plus répandus. Au travers d’applications variées (environnement, chimie fine, pharmaceutique, etc..), nous découvrions leurs possibilités concrètes, leurs points communs, leurs spécificités et ce qu’ils peuvent vous apporter dans la vie de tous les jours au laboratoire.

Optimizing column handling on the HPLC
Ruedi Schwarz, sertec electronics

Today you have to change your column more and more often to adapt your instrument to the different application demands. Your work pressure is increasing from year to year. And according to Murphy’s Law, your sequence will not start or is interrupted due to a leak within your column compartment at the time when you’re already in a hurry.
With the introduction of shorter columns the dead volume of the connections becomes more and important. With OPTI-LYNX or even the VELOSITY Program you are changing your column extremely fast and secure.

ms-NoVent II - what is new?
Bernd Pfeffer, SGE (Deutschland) GmbH Stand: infochroma ag

MS-NoVent is a device, which allows the fast exchange of capillaries in GC/MS-Systems without the need to vent the MS. This is successfully performed by a direct stream of
Helium and is well tried. The ms-NoVent has now been further developed. What advantages result from this product development? Learn about the new details, which make this good product even better and even more user friendly.

Developments in SPME

Metal Fibers, improved coating process, new DIN-Method for Pesticide Analysis applying SPME
Since its introduction in the early nineties the Solid Phase Micro Extraction (SPME) has gained more and more importance in laboratories. Due to its simple handling and quick results, the SPME has become a key sample preparation technique for GC even more as it can be automated for unattended analysis. To improve the performance and durability Supelco has introduced superelastic metal fibers that show more mechanical stability and therefore longer lifetimes. In addition a new coating process has been developed resulting in more even and reproducible fiber coating.
In May 2006 the DIN published a method (DIN 38407-34) for the determination of selected pesticides, biocides and metabolites via GC-MS and SPME.
The workshop will provide an overview on this method as well as the new metal fibers and the improved coating process.

Columns and Solvents for LC/MS
Klaus Buckendahl, Sigma-Aldrich Chemie GmbH

Alternative selectivity to C18, Low-Bleed Polar-Embeded RP-Phase, High Purity Solvents
In HPLC commonly C18 is the most used phase and also first choice for a column in method development. However C18 might not be the best phase for an analysis and an alternative selectivity would provide quicker and more reliable results with a simpler method. Polar-Embedeed phases are such an alternative. Due to their construction these phases suffered in the past from bleeding making these phases only limited to not suitable for LC/MS. With the Ascentis RP-Amide is now a low bleed polar embeded phase available suitable for LC/MS.
Additional to the right HPLC phase/columns the mobile phase can plays an important role in LC/MS. Background noise and interferences can be caused by the use of not suited solvents and additives. The Chromasolv LCMS solvents are specifically tested and packaged for the use in demanding applications in LC/MS.
This workshop will provide information on the use, properties and benefits of the low-bleed polar embeded HPLC phase and the LC/MS solvents.

Varian 1200 GC-MS QQQ
Karl Abele, Varian AG
 
Exposition

www.bgb-analytik.ch
sales@bgb-analytik.ch

- Raccords et Diviseurs PressFit
- Colonnes et Accessoires pour CPG et CPL
- Tube de silicefondue
- Seringues
- Filtres pour seringues et membranes
- Flacons et Accessoires
Représentation: ATAS, CRS, CTC, GL Science, Hamilton, Heraeus/Cathodeon/IST, Kinesis, National Scientific, Restek, SGE, Vici/Valco

www.brechbuehler.ch
sales@brechbuehler.ch

Thermo GC et GC/MS et autosampler
- Phenomenex colonnes HPLC et GC et cartouches SPE
www.camag.com
info@camag.com

- ADC2
- Digistore 2
www.dionex.ch
dionex@dionex.ch
- UltiMate 3000 LC and LC/MS Systems
- Chromeleon Chromatography Data System
www.infochroma.ch
info@infochroma.ch
- programme de flacons étendu
- filtres HPLC
- Seringues, Liners et Fittings
- Colonnes Capillaires GC de SGE
- Colonnes HPLC Brownlee et Column Engineering
- Colonnes Chiral Astec
- Colonnes Shodex
- WebSeal pour microtiter plates 96 et 384
- 3M Vacuum Disk & Disk Manifold
- Agilent Technologies Colonnes et Accessoires

www.igz.ch
igz@igz.ch

- CombiFlash Companion 4X
www.mt.com/autochem
info.ch@mt.com
- Chromatographe en phase supercritique (SFC)
www.milian.com
milian@milian.com
- Agilent: colonnes et accessoires
- Hamilton: seringues, dilutors et colonnes
- Omnifit
www.stehelin.com
service@stehelin.ch
- Solvant (HPLC, LCMS) de JT Baker
- SPE
- Colonnes HPLC
- Colonnes GC
www.perkinelmer.com
swiss.info@perkinelmer.com
- TMX ATD & HS Trap
- Jade DSC
- Spectrum 100 (FTIR)
www.sertec.ch
info@sertec.ch
HPLC Specialities
- OPTI-LYNXTM Column Connector Kit
- VELOCITY® Column System
- OPTI-SOLVTM Mini Filter
- OPTI-MAX® Check Valve
Detector Lamp
- Hollow Cathode Lamps
- Deuterium Lamps
- Xenon Lamps

Epson Products

www.sigma-aldrich.com
fluka@sial.com

- Nouvelles ASCENTIS HPLC colonnes de Supelco
- Low Bleed Capillary GC Colonnes
- VersaFlash chromatographie système
- SPME New Application Guide, 5ième edition
- Vials & More
- Standards analytiques de Fluka & Riedel de Haën
- HYDRANAL Karl Fischer Titration
www.varianinc.com
analytical.ch@varianinc.com
- Varian 1200 GC-MS QQQ

  infoExpo Organisations-Büro: infochroma ag, Sumpstr. 3, CH-6300 Zug, info@infoexpo.ch